Key result
The interaction of magnesium-ADP with skeletal muscle heavy meromyosin releases about 0.5 mol of protons per mol of protein at pH 8.0 and 25 degrees C, with a binding constant of 2 X 10(5) M-1.
Population
Skeletal muscle heavy meromyosin
Design
Preclinical
Authors
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Skeletal muscle findings warrant no clinical extrapolation; leaves open cardiac myosin binding mechanisms for further study.
The study characterizes the binding equilibria of magnesium-ADP to skeletal muscle heavy meromyosin through proton release measurements, revealing pH-dependent association constants and pK shifts.
Marsh et al. (1977) studied this question. Magnesium-ADP vs. Potassium-ADP was evaluated on Proton release upon binding. The interaction of magnesium-ADP with skeletal muscle heavy meromyosin releases about 0.5 mol of protons per mol of protein at pH 8.0 and 25 degrees C, with a binding constant of 2 X 10(5) M-1.
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