Key result
Mutations Asn796-->Ala and Thr799-->Ala abolished Ca2+ occlusion, indicating their side chains are assigned to two different Ca2+ sites involved in occlusion, unlike Glu908.
Population
Sarcoplasmic reticulum Ca(2+)-ATPase mutants (Asn796-->Ala, Thr799-->Ala, and Glu908-->Ala)
Comparison
Mutagenesis vs Comparison among mutants and wild-type properties
Design
Preclinical
Authors
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Identifies distinct Ca2+ ligands in SERCA; leaves open therapeutic targeting in cardiac dysfunction.
Asn796 and Thr799 are involved in Ca2+ occlusion at different sites in the sarcoplasmic reticulum Ca(2+)-ATPase, whereas Glu908 is not a direct Ca2+ ligand.
Andersen et al. (1994) studied this question. Mutants Asn796-->Ala, Thr799-->Ala, and Glu908-->Ala of the sarcoplasmic reticulum Ca(2+)-ATPase was evaluated on Ca(2+)-binding properties, Ca2+ occlusion, and phosphorylation. Mutations Asn796-->Ala and Thr799-->Ala abolished Ca2+ occlusion, indicating their side chains are assigned to two different Ca2+ sites involved in occlusion, unlike Glu908.
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