Why the study?
Does the choice of extraction technique affect the measured plasma angiotensin I and II levels in patients treated with enalapril?
Does the choice of extraction technique affect the measured plasma angiotensin I and II levels in patients treated with enalapril?
The study highlights the importance of selecting the appropriate extraction technique to prevent in vitro conversion of angiotensin I to II during radioimmunoassay in patients treated with ACE inhibitors.
Choice of extraction technique may artifactually alter measured AI/AII levels during enalapril therapy; leaves open optimal assay standardization for clinical and research use.
Plasma angiotensin I and II (AI and AII) were measured in 13 patients with essential hypertension before and during chronic treatment with enalapril (MK 421) and in seven subjects during an acute study. Two techniques were used for simultaneous extraction of AI and AII. Despite appropriate correction for cross-reaction of AI with the AII antibody, one of the techniques gave consistently higher AII and lower AI levels in plasma of subjects treated with enalapril. The possibility of in vitro conversion of AI into AII-immunoreactive material during the purification or the radioimmunoassay steps should be considered. The use of the alternative technique is proposed for simultaneous processing of blood samples for AI and AII.
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Boer et al. (1985) studied this question.
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