What question did this study set out to answer?

This study aims to investigate the role of NBCe1 in cardiac myocytes during ischemia-reperfusion injury.

September 30, 2018World Journal of CardiologyOpen Access

NBCe1 Na+-HCO3- cotransporter ablation causes reduced apoptosis following cardiac ischemia-reperfusion injury in vivo

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Why the study?

Does NBCe1 Na+-HCO3- cotransporter ablation reduce apoptosis following cardiac ischemia-reperfusion injury in vivo?

Population

Animal model subjected to in vivo cardiac ischemia-reperfusion injury

Comparison

Ablation of NBCe1 Na+-HCO3- cotransporter in… vs Wild-type (WT) controls

Design

Preclinical

Follow-up

3 hours of reperfusion

Authors

KVKanimozhi VairamaniCincinnati Children's Hospital Medical Center VPVikram PrasadCincinnati Children's Hospital Medical Center YWYigang WangCollaborative Innovation Center of Advanced Microstructures

Discussion

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Implication

NBCe1 ablation may limit post-I/R apoptosis in mice; hypothesis-generating and should not yet change practice.

Key Points

This study aims to investigate the role of NBCe1 in cardiac myocytes during ischemia-reperfusion injury.
Analyzed ischemia-reperfusion injury after 30 min occlusion of the left anterior descending artery followed by 3 h of reperfusion.
Compared knockout (KO) and wild-type (WT) heart sections for cellular apoptosis and gene expression changes.
KO heart sections exhibited significantly fewer apoptotic nuclei compared to WT sections after ischemia and reperfusion.

Structured PICO

Does NBCe1 Na+-HCO3- cotransporter ablation reduce apoptosis following cardiac ischemia-reperfusion injury in vivo?

Population

Animal model subjected to in vivo cardiac ischemia-reperfusion injury (30 min occlusion of the left anterior descending artery)

Intervention

Ablation of NBCe1 Na+-HCO3- cotransporter in cardiac myocytes (KO)

Comparator

Wild-type (WT) controls

Outcome

Apoptosis (number of apoptotic nuclei) following ischemia and reperfusionsurrogate

Ablation of the NBCe1 Na+-HCO3- cotransporter in cardiac myocytes reduces apoptosis following ischemia-reperfusion injury in vivo without impairing basal or stimulated cardiac function.

Cite This Study

Vairamani et al. (2018) studied this question.

synapsesocial.com/papers/6a237a3996b50e6ae79ece4c https://doi.org/10.4330/wjc.v10.i9.97

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NBCe1 Na+-HCO3- cotransporter ablation causes reduced apoptosis following cardiac ischemia-reperfusion injury in vivo

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

NBCe1 Na+-HCO3- cotransporter ablation causes reduced apoptosis following cardiac ischemia-reperfusion injury in vivo

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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