What is the clinical evidence from this study?

Study design: Other. Population: Ischemia. Intervention: HOE-642 vs. Control. Primary outcome: Postischemic recovery of left ventricular developed pressure (LVDP) after 40 minutes of ischemia (p=<.05).

What question did this study set out to answer?

To determine the role of Na+/H+ exchanger activity in ischemic preconditioning's cardioprotective effects in isolated rat hearts.

November 18, 1997Circulation

Na+/H+Exchanger Activity Does Not Contribute to Protection by Ischemic Preconditioning in the Isolated Rat Heart

Q: What are the key findings of this study?

HOE-642 improved postischemic recovery of left ventricular developed pressure to 45% vs 15% in controls (P<.05) and enhanced the protective effect of ischemic preconditioning in isolated rat hearts.

Q: What question did this study set out to answer?

To determine the role of Na+/H+ exchanger activity in ischemic preconditioning's cardioprotective effects in isolated rat hearts.

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Key result

HOE-642 improved postischemic recovery of left ventricular developed pressure to 45% vs 15% in controls (P<.05) and enhanced the protective effect of ischemic preconditioning in isolated rat hearts.

Population

Isolated rat ventricular myocytes and isolated rat hearts

Comparison

HOE-642, an NHE inhibitor, infused for 5 minutes… vs Control, ischemic preconditioning alone, or…

Design

Preclinical

Authors

ASAlex ShipoliniSt Bartholomew's Hospital HYHiroyuki YokoyamaHyogo Medical University MGManuel GaliñanesHebron University

Discussion

Member takes

Implication

NHE inhibition may enhance experimental preconditioning; leaves open clinical translation in ischemia-reperfusion.

Key Points

To determine the role of Na+/H+ exchanger activity in ischemic preconditioning's cardioprotective effects in isolated rat hearts.
Involved 8 to 11 isolated rat ventricular myocytes per group using the fluorescent pH indicator C-SNARF-1.
Measured left ventricular developed pressure (LVDP) recovery after ischemia with NHE inhibition using HOE-642.
Conducted two protocols: one with NHE inhibition during prolonged ischemia and another before ischemic preconditioning.
LVDP recovery was 68±2% for combined ischemic preconditioning and NHE inhibition versus 34±5% for ischemic preconditioning alone (P<.05).
NHE inhibition resulted in significant improvements in LVDP recovery compared to controls for 60 minutes of ischemia.
Creatine kinase leakage was reduced with NHE inhibition showing improved recovery outcomes.

Structured PICO

Population

Isolated rat ventricular myocytes and isolated rat hearts subjected to prolonged ischemia and ischemic preconditioning.

Intervention

HOE-642 (1 micromol/L), an NHE inhibitor, infused for 5 minutes before prolonged ischemia or for 3 minutes before ischemic preconditioning episodes

Comparator

Control (no intervention), ischemic preconditioning alone, or HOE-642 alone

Outcome

Postischemic recovery of left ventricular developed pressure (LVDP)surrogate

Main Result

Absolute Event Rate: 45% vs 15%

p-value: p=<.05

In an isolated rat heart model, NHE inhibition with HOE-642 did not reduce the efficacy of ischemic preconditioning and enhanced protection during prolonged ischemia.

Cite This Study

Shipolini et al. (1997) studied Ischemia. HOE-642 vs. Control was evaluated on Postischemic recovery of left ventricular developed pressure (LVDP) after 40 minutes of ischemia (p=<.05). HOE-642 improved postischemic recovery of left ventricular developed pressure to 45% vs 15% in controls (P<.05) and enhanced the protective effect of ischemic preconditioning in isolated rat hearts.

synapsesocial.com/papers/6a237a3d96b50e6ae79ece71 https://doi.org/10.1161/01.cir.96.10.3617

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Na+/H+Exchanger Activity Does Not Contribute to Protection by Ischemic Preconditioning in the Isolated Rat Heart

Discussion

Member takes

Implication

Key Points

Structured PICO

Main Result

Cite This Study

Na+/H+Exchanger Activity Does Not Contribute to Protection by Ischemic Preconditioning in the Isolated Rat Heart

Discussion

Member takes

Implication

Key Points

Structured PICO

Main Result

Cite This Study

Also Consider

Also Consider