What question did this study set out to answer?

This research aims to develop an effective method for isolating and purifying osteocytes from bone.

Isolation and purification of osteocytes

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Isolation method produces highly pure osteocytes, indicating potential for further studies.

This research aims to develop an effective method for isolating and purifying osteocytes from bone.
Isolated bone cells from calvariae of 18-day-old chicken embryos using collagenase and EDTA.
Purified osteocytes with osteocyte-specific MAb OB 7.3 attached to magnetic beads.
Achieved over 95% purity in the osteocyte population.
Isolated osteocytes adhered rapidly to substrates and exhibited various processes.
After 1-2 days in culture, osteocytes formed a network similar to natural bone structures.

Plas et al. (1992) studied this question.

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