What question did this study set out to answer?

To isolate viable osteocytes and characterize their morphology, enzymatic profile, parathyroid hormone responsiveness, and potential role in matrix resorption in vitro.

November 1, 1994Journal of Bone and Mineral ResearchOpen Access

Characteristics and properties of osteocytes in culture

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Authors

APA. van der PlasRijnland Ziekenhuis EAE.M. AardenLeiden University JFJan Feijen

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Implication

In vitro study reveals that cultured osteocytes respond to parathyroid hormone without resorbing bone in chick calvariae, indicating an endocrine sensory role rather than direct osteolysis.

Key Points

To isolate viable osteocytes and characterize their morphology, enzymatic profile, parathyroid hormone responsiveness, and potential role in matrix resorption in vitro.
Isolated bone cell populations from fetal chick calvariae through sequential enzymatic and chelating treatments using collagenase and EDTA.
Purified osteocytes via immunomagnetic separation employing monoclonal antibody MAb OB 7.3 conjugated to magnetic beads.
Assayed cultured osteocytes for alkaline phosphatase activity, parathyroid hormone-induced cyclic AMP synthesis, and surface corrosion on dentin slices.
Purified osteocytes exhibited a postmitotic, stellate morphology and demonstrated lower, highly heterogeneous alkaline phosphatase activity compared with osteoblast-like cells.
Osteocytes expressed parathyroid hormone receptors and accumulated intracellular cyclic AMP upon stimulation, displaying greater hormone sensitivity than osteoblasts.
Cultured osteocytes produced no measurable corrosion pits when cultured on dentin slices, providing no evidence of osteocytic osteolysis.

Cite This Study

Plas et al. (1994) studied this question.

synapsesocial.com/papers/6a763d41dd478a52beb1b741 https://doi.org/10.1002/jbmr.5650091105

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Implication

In vitro study reveals that cultured osteocytes respond to parathyroid hormone without resorbing bone in chick calvariae, indicating an endocrine sensory role rather than direct osteolysis.

Characteristics and properties of osteocytes in culture

Discussion

Member takes

Implication

Key Points

Cite This Study

Characteristics and properties of osteocytes in culture

Discussion

Member takes

Implication

Key Points

Cite This Study

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