Evaluation of antibacterial effects of Eucalyptus camaldulensis L., extracts against some pathogenic bacteria in vitro and HPLC-DAD

Our study was carried out in laboratory of Alnoor university –Iraq in ( 2025) to identify active compounds and evaluate in vitro antimicrobial which include effects of hexane and aquatic extracts with three concentration on two species of bacteria , the phenolic compounds for Eucalyptus camaldulensis tree identify active compounds extract was prepared by using Soxhlet hexane and Aqueous as a solvent HPLC High performers liquid Chromatography , was used to diagnose and identify phenolic compounds in Eucalyptus extract. Results showed identification of 5 active phenolic compounds, (Quercetin, Kaempferol, Ferulic, Gallic acid, Rutine). These differences in chemical compounds could be used as an important indicator for plant identification. The second aspect of study investigated inhibitory effect of two organic solvents on two bacterial species (Staphylococcus aureus and Escherichia coli). Research revealed that inhibition rates varied depending on solvent used. Furthermore, the in vitro antimicrobial potential of E. Camaldulensis extracts demonstrated varying degrees of inhibition at different concentrations, which also differed between Gram-positive and negative bacteria.