Molecular Diagnosis of Enterobacter Cloacae and Stutzerimonas Stutzeri and Study of Effect of Some Plant Extract

Periodontal pockets are thought to provide spawning grounds for a variety of microorganisms. Emergence of antibiotic-resistant bacterial strains poses a risk on course of treatment and may require surgical removal of gum pocket. This study's aim was to determine the bacteria found in gum pockets using PCR for 16S rRNA gene. As well as impact of plant extracts on growth of these strains. Five new bacterial strains have been registered at NCBI, including Stutzerimonas stutzeri, Staphylococcus aureus, and Enterobacter cloacae. All identified strains were susceptible to Augmentin and Ciprofloxacin but resistant to Cephalothin. Cefixime and Bacitracin were not effective against 90% of strains. Amoxicillin resistance rate was 50%. All strains were resistant to Turmeric and Oregano extracts at concentrations of 100 and 200 mg/ml, with exception of Staph. aureus FKZEW99, which was susceptible to Oregano at 200 mg/ml. Identified strains' responses to clove extract differed in terms of sensitivity and resistance. The overuse of antibiotics leads to appearance of new bacterial strains resistant to a range of treatments.