Platelet Proteome Reveals Novel Targets for Hypercoagulation in Pseudoexfoliation Syndrome

Figures

• 

  Figure 1. Parameters of Extem test in PEX and RVO patients, and the controls. Each parameter is depicted in a different panel. (A) Clotting time (CT). (B) Clot formation time (CFT). (C) Alpha angle. (D) A10 (amplitude at the 10th minute). (E) A20 (amplitude at the 20th minute). (F) Maximum clot firmness (MCF). * p < 0.05.

• 

  Figure 2. Parameters of Intem test in PEX and RVO patients, and the controls. Each parameter is depicted in a different panel. (A) Clotting time (CT). (B) Clot formation time (CFT). (C) Alpha angle. (D) A10 (amplitude at the 10th minute). (E) A20 (amplitude at the 20th minute). (F) Maximum clot firmness (MCF). * p < 0.05.

• 

  Figure 3. Parameters of Fibtem test in PEX and RVO patients, and the controls. Each parameter is depicted in a different panel. (A) Clotting time (CT). (B) Clot formation time (CFT). (C) A10 (amplitude at the 10th minute). (D) A20 (amplitude at the 20th minute). (E) Maximum clot firmness (MCF).

• 

  Figure 4. The surface expression of platelet markers in control group, pseudoexfoliation (PEX) patients, and retinal vein occlusion (RVO) patients. The relative expression levels of CD41a (A), CD42b (B), CD61 (C), and CD62p (D) markers are shown.

• 

  Figure 5. Bioinformatic analysis of protein–protein interactions of differentially expressed proteins in platelets of PEX and RVO patients, and the controls. The abbreviations of protein names are provided in Table 1, except ACTB and HSCB, which refer to beta-actin and iron–sulfur cluster co-chaperone protein, respectively. Pink and light-blue lines confirm known interactions, green and dark-blue lines indicate predicted interactions, and black lines indicate the co-expression between proteins http://www.string-db.org (accessed on 31 October 2023).

• 

  Figure 6. Profilin-1 expression levels in PEX and RVO patients, and the controls. Images show Profilin-1 and GAPDH expression in a representative group of patients and controls. Profilin-1 expression levels were normalized to GAPDH. The graph shows the change in relative protein expression in all studied subjects. * p < 0.001.

• 

  Figure 7. Plasma levels of endothelial activation markers in PEX and RVO patients, and the controls. (A) P-selectin levels, (B) E-selectin levels, and (C) von Willebrand factor (vWF) levels in study groups. * p < 0.05.