The exosome of platelet endothelial cell adhesion molecule-1 (PECAM1) protein

Figures

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  Figure 1. The protein PECAM1 in the exosomes harvested from the blood of high blood pressure patients. (A) The derived exosomes imaged with NTA at a diameter in the range of 50 to 100 nm. Scale bar, 100 nm. (B) Arrows indicate PECAM1 immunogold labeling (black dots) on the exosome surface. Scale bars, 100 nm. PECAM1 = platelet endothelial cell adhesion molecule-1.

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  Figure 2. The surface marker protein was extracted from exosomes and analyzed with western blot, the results revealed that the exosomal markers CD81 and CD63 were obiviously expressed on the exosome surface (samples: S1–S8).

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  Figure 3. The expression of PECAM1 in the exosome of the blood samples were analyzed by Western Blot (A: the representative samples in the high blood patients vs the healthy control) (high blood patients’ samples: Group 1–4: A1-B1-C1-D1; healthy control's samples: Group 1–4: E1-F1-G1-H1). And the difference was analyzed with GraphPad Prism 6.0 software, (B) in the 4 representative groups, the expression of PECAM1 protein in the exosome was higher than the healthy controls, ∗, &, # represented with significance (P < .05). PECAM1 = platelet endothelial cell adhesion molecule-1.

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  Figure 4. The correlation between the expression PECAM1 in the exosome and the systolic pressure of the high blood pressure patients, the expression PECAM1 was higher in high blood pressure patients and there was significant correlation in each patient’ blood sample, the linear coefficients R = 0.901, P < .01. PECAM1 = platelet endothelial cell adhesion molecule-1.