Characterization of inactive renin from human kidney and plasma. Evidence of a renal source of circulating inactive renin.
Population
Human kidney cortex tissue and plasma samples, including blood samples from 8 consecutive patients
Design
Preclinical
Authors
Discussion
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Implication
Supports renal origin of plasma inactive renin in humans; leaves open its physiological and clinical roles.
Key Points
- This investigation aims to isolate and characterize inactive renin from human kidney and plasma, highlighting its renal origin.
- Rapid processing of human kidney cortex at temperatures below 4 degrees C with protease inhibitors
- Cibacron-blue affinity chromatography for extraction
- Simultaneous measurement of inactive renin in renal veins and inferior vena cava in eight patients.
- Inactive renin constitutes 10 to 50% of total renal renin concentration.
- Inactive renin from plasma and kidney has a pH optimum of activation at 3.3 and similar enzymatic properties.
- Concentration of inactive renin is significantly higher in renal venous blood compared to inferior vena caval blood.
Structured PICO
P
Population
Human kidney cortex tissue and plasma samples, including blood samples from 8 consecutive patients
I
Intervention
Biochemical characterization and measurement of inactive renin
O
Outcome
Biochemical properties and concentration of inactive renin in renal venous blood versus inferior vena caval bloodsurrogate
The human kidney contains and secretes significant quantities of inactive renin, establishing it as a major source of circulating inactive renin in human plasma.
Cite This Study
Hsueh et al. (1983) studied this question.