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This research investigates the catalytic role of ACAT-1 protein across various human tissues.

Immunodepletion experiments suggest that acyl-coenzyme A:cholesterol acyltransferase-1 (ACAT-1) protein plays a major catalytic role in adult human liver, adrenal gland, macrophages, and kidney, but not in intestines

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Immunodepletion experiments reveal ACAT-1's significant catalytic role in major organs, excluding intestines.

This research investigates the catalytic role of ACAT-1 protein across various human tissues.
Used anti-ACAT-1 antibodies for immunodepletion of ACAT-1 from human tissue homogenates.
Measured residual ACAT activity in supernatants to evaluate functional significance.
Examined ACAT activity levels in liver, adrenal gland, macrophages, kidney, and intestines.
ACAT activity immunodepleted by 90% in liver (83% in hepatocytes) and 98% in adrenal gland.
ACAT activity decreased by 91% in macrophages and 80% in kidney; only 19% in intestines.
Intestinal ACAT activity resistant to immunodepletion, more sensitive to ACAT inhibitor Dup 128.

Lee et al. (1998) studied this question.

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