What does this research mean for the field?

A multiplexable flow cytometry protocol enables the simultaneous high-throughput quantification of antibody binding to up to three native-like, cell-surface-expressed viral antigens per well across 500 samples within three days. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

The aim is to develop a protocol for efficiently measuring antibody binding to viral proteins, specifically hemagglutinin.

June 7, 2026STAR ProtocolsOpen Access

Protocol for multiplexed detection of antibody binding to cell-surface-expressed hemagglutinin by flow cytometry

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Authors

LULeon UllrichUniversity of Cologne KDKatharina DanielUniversity of Cologne ASAnna-Lena SchumacherMax Planck Institute for Biology of Ageing

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Implication

Protocol demonstrates multiplexing antibody detection of hemagglutinin in human cells, suggesting a tool for monitoring immunity.

Key Points

The aim is to develop a protocol for efficiently measuring antibody binding to viral proteins, specifically hemagglutinin.
Transfection of human suspension cells with hemagglutinin
Cell staining and fixation for flow cytometry
Simultaneous measurement of up to 500 samples per run against three antigens.
Successfully quantified antibody binding to native-like hemagglutinin
Demonstrated ability to analyze multiple samples efficiently within three days
Optimized protocol for high-throughput analysis of cell-surface-expressed antigens.

Cite This Study

Ullrich et al. (2026) studied this question.

synapsesocial.com/papers/6a250a3c7def13d035e1a67c https://doi.org/10.1016/j.xpro.2026.104614

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Protocol for multiplexed detection of antibody binding to cell-surface-expressed hemagglutinin by flow cytometry

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Key Points

Cite This Study

Protocol for multiplexed detection of antibody binding to cell-surface-expressed hemagglutinin by flow cytometry

Discussion

Member takes

Implication

Key Points

Cite This Study

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