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August 1, 1944The Journal of Immunology

A Simplified Procedure for Titrating Hemagglutinating Capacity of Influenza-Virus and the Corresponding Antibody

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Authors

JSJonas SalkBlaze Bioscience (United States)

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Implication

Laboratory study demonstrates a simplified hemagglutination assay for influenza virus and serum antibodies, suggesting improved efficiency and reduced reagent requirements.

Key Points

  • Develop and standardize a simplified modification of Hirst's hemagglutination test to quantify influenza virus hemagglutinating capacity and serum antibody titers.
  • Modified Hirst's assay by decreasing erythrocyte volume, determining endpoints via sedimentation patterns, and removing rigid incubation time restrictions.
  • Assessed the effects of temperature, incubation duration, erythrocyte concentration, and reagent addition sequence to optimize assay conditions.
  • Simplified serum antibody testing by preparing serial serum dilutions directly within the red blood cell suspension.
  • Standardized assay criteria improved diagnostic sensitivity while reading sedimentation patterns eliminated the need for strict timing intervals.
  • Direct dilution of serum within erythrocyte suspensions reduced total required reagents and halved reagent addition steps.

Cite This Study

Jonas Salk (1944) studied this question.

synapsesocial.com/papers/6a6ce98226a7f98052db5734https://doi.org/10.4049/jimmunol.49.2.87
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Also Consider

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  1. 1IMMUNE ADHERENCE HEMAGGLUTINATION TEST AS A NEW SENSITIVE METHOD FOR TITRATION OF ANIMAL VIRUS ANTIGENS AND ANTIBODIES1966 · 26 citations
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  3. 3Protocol for multiplexed detection of antibody binding to cell-surface-expressed hemagglutinin by flow cytometry2026
  4. 4Hemagglutination by Measles Virus. 4. A Simple Procedure for Production of High Potency Antigen for Hemagglutination-Inhibition (HI) Tests1962 · 216 citations
  5. 5Changes in antibody avidity after virus infections: detection by an immunosorbent assay in which a mild protein-denaturing agent is employed1984 · 139 citations