2895-LB: Continuous Lactate Sensing

Introduction and Objective: Mitochondria are known as the powerhouse of the cell, providing primary ATP energy for most cellular functions. Lactate is an important biomarker acting as a dynamic indicator of metabolic efficiency, oxidative capacity, and when chronically elevated, metabolic dysfunction. A sedentary lifestyle has been shown to decrease the number, size, and function of mitochondria in skeletal muscle. A continuous lactate sensor can be used to provide dynamic feedback on positive lifestyle changes, such as regular exercise which is the best-known method to improve mitochondrial function. With accuracy and low latency, such a sensor enables precise exercise prescription based on true metabolic response rather than external workload alone. By continuously monitoring lactate kinetics, it is possible to individualize training intensity, identify optimal training zones, assess lactate clearance rates and track improvements in mitochondrial function over time. Methods: Forty intradermal lactate sensors were placed on the forearms of ten healthy subjects. Each subject performed an exercise challenge on a stationary bike with increasing resistance and capillary lactate samples (Nova Biomedical, Lactate Plus) every 3-5 minutes. This test was repeated each day for three days. Sensor output was evaluated for latency by assessing the timing of both initial lactate rise and maximum lactate peak against concurrent comparators. Results: Sensors were evaluated for signal quality during each of the daily sessions and the sensor identification time of a rise in tissue lactate was 1.5, -0.8, -1.7 minutes, respectively by day, compared to the concurrent blood sample (average -0.3, n=92). The peak lactate was identified by the sensor 2.4 minutes on average after the peak blood lactate (1.8, 2.5, 2.9). Each identified feature was within the 3-minute blood sampling time, which suggests that the dermal sensor has minimal latency compared to blood. Conclusion: This study demonstrated that an intradermal continuous lactate sensor has extremely low latency with dynamic lactate changes during exercise. Disclosure R. Gottlieb: Employee; Current; Biolinq. C.A. Silva Lopez: None. A. Warren: None. B. Wang: Employee; Current; Biolinq. I. San Millan: Advisory Panel; Current; Biolinq. J.R. Tangney: Employee; Current; Biolinq. Funding National Institutes of Health (2R44DK129875-04)