Why the study?
Does a neutralization enzyme immunoassay (N-EIA) correlate with the standard hemagglutination inhibition test for detecting antibody titer rises in patients infected with influenza A (H3N2) virus?
Does a neutralization enzyme immunoassay (N-EIA) correlate with the standard hemagglutination inhibition test for detecting antibody titer rises in patients infected with influenza A (H3N2) virus?
A newly developed neutralization enzyme immunoassay correlates well with the standard hemagglutination inhibition test for detecting influenza A (H3N2) antibodies.
N-EIA may offer a lab alternative for influenza A serology; leaves open prospective validation before clinical adoption.
A neutralization enzyme immunoassay (N-EIA) was developed for the detection of antibody titer rises in sera of patients infected with influenza A (H3N2) virus. In this N-EIA, a selected strain of influenza A (H3N2) virus was added to monolayers of LLC-MK2 cells in microtiter plates. After 24 h, the replicated virus could be demonstrated with a virus-specific enzyme-labeled monoclonal antibody. Preincubation of the influenza virus with convalescent-phase sera of patients infected with influenza A (H3N2) virus resulted 1 day later in decreased absorbance values that could be used for calculation of neutralization titers. From use of paired serum samples from 10 patients with a history of flu-like symptoms, the results obtained with N-EIA correlated well (r = 0.83) with those of the standard hemagglutination inhibition test.
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Benne et al. (1994) studied this question.
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