Profiling the Chlorogenic Acids and Other Caffeic Acid Derivatives of Herbal Chrysanthemum by LC−MSn

Four samples of herbal chrysanthemum have been profiled qualitatively by LC−MS5 to identify their component chlorogenic acids and partially characterize other caffeic acid derivatives. The chlorogenic acids were minor components, and the four samples varied markedly in profile. Three p-coumaroylquinic acids, three feruloylquinic acids, four caffeoylquinic acids, six dicaffeoylquinic acids, and two tricaffeoylquinic acids were detected, 13 for the first time from this source. Partial characterization of minor components suggested the presence of five caffeoyl-hexose esters and caffeic acid-4-β-d-glucose that have not previously been reported from this source, and eight caffeoylquinic acid glycosides and 16 dicaffeoylquinic acid glycosides that have not previously been reported in nature. Succinic acid-containing chlorogenic acids and chlorogenic acids based on epi-quinic acid, previously reported in Chrysanthemum spp., were not detected in these samples. Keywords: Asteraceae; Beijuhua; caffeic acid glycosides; caffeoyl-hexoses; caffeoylquinic acids; caffeoylquinic acid glycosides; chlorogenic acids; Chrysanthemum; p-coumaroylquinic acids; cynarin; Dendranthema; dicaffeoylquinic acid glycosides; dicaffeoylquinic acids; disuccinoyl-caffeoylquinic acids; feruloylquinic acids; Garland; Gongju; Ju Hua; LC−MSn; succinoyl-caffeoylquinic acids; succinoyl-dicaffeoylquinic acids; tricaffeoylquinic acids