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June 10, 2026Biotechnology and Bioengineering

An Antibiotic‐Free Cryptic Plasmid Platform for Modular Pathway Coupling in Probiotic Escherichia Coli Nissle 1917

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Authors

XCXue CaiJCJie CuiLXLu Xiong

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Overview

Randomized trial demonstrates enhanced vitamin B5 production in probiotic E. coli, indicating its potential for value-added metabolites.

Key Points

  • This research aims to develop a stable, antibiotic-free platform for vitamin B5 biosynthesis in probiotic E. coli Nissle 1917.
  • Integrated global regulatory rewiring with a cryptic plasmid-based pathway coupling platform
  • Deletion of global transcriptional regulators CRP and CRA to reshape carbon metabolism
  • Engineering of native cryptic plasmids for tunable gene expression and stability.
  • The engineered strain produced 98 mg/L vitamin B5 in shake-flask cultures with stable productivity over multiple passages.
  • In a 5-L fed-batch bioreactor, vitamin B5 titer reached 1.15 g/L, an 11.8-fold improvement over flask cultivation.

Cite This Study

Cai et al. (2026) studied this question.

synapsesocial.com/papers/6a28ff336f82f25be989c38bhttps://doi.org/10.1002/bit.70254
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Construction of Probiotic Escherichia coli Nissle 1917 as a Next- Generation Expression Platform and Its Application in Therapeutic Protein Production2025
  2. 2Multilevel Metabolic Engineering of Probiotic <i>Escherichia coli</i> Nissle 1917 for Antibiotic-Free Production of β-Alanine2026
  3. 3Sequential Plasmid Curing and Genome Editing in <i>Escherichia coli</i> Nissle 19172026
  4. 4Escherichia coli Nissle 1917 as a Probiotic Microbial Cell Factory: From Genetic Engineering to Fermentation2026 · 2 citations
  5. 5Engineering Escherichia coli Nissle as safe chassis for delivery of therapeutic peptides2025