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June 13, 2026ACS Synthetic Biology

Multilevel Metabolic Engineering of Probiotic Escherichia coli Nissle 1917 for Antibiotic-Free Production of β-Alanine

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Authors

XZXi ZhangXWXuyun WangZLZi-Hang Li

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Overview

Randomized trial enhances β-alanine biosynthesis in Escherichia coli, suggesting a new method for antibiotic-free production.

Key Points

  • The aim is to enhance β-alanine biosynthesis using multilevel metabolic engineering in probiotic Escherichia coli Nissle 1917.
  • Introduced heterologous key enzymes for β-alanine production
  • Reinforced the precursor L-aspartate supply through dual-pathway approach
  • Engineered transport systems and modulated competing pathways
  • Produced 60.55 g/L β-alanine with a yield of 0.30 g/g glucose
  • Achieved the highest synthesis of β-alanine in probiotic E. coli to date
  • Examined functions of several proton-driven symporters related to β-alanine accumulation

Cite This Study

Zhang et al. (2026) studied this question.

synapsesocial.com/papers/6a2cf36efaef96ed7f055e06https://doi.org/10.1021/acssynbio.6c00228
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Also Consider

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