A broadly reactive TaqMan assay targeting the 5'-untranslated region of the hepatitis A virus detected 0.5 infectious units of HAV and 40 copies of a synthetic transcript.
The developed TaqMan assay provides a rapid and sensitive screening tool for quantitative HAV detection in clinical or environmental samples.
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Primers and a TaqMan probe for the 5'-untranslated region (UTR) of the hepatitis A virus (HAV) genome were designed and evaluated. The assay detected 0.5 infectious units of HAV and 40 copies of a synthetic transcript and provides an important screening tool for rapid quantitative HAV detection in clinical or environmental samples.
Jothikumar et al. (Wed,) reported a other. A broadly reactive TaqMan assay targeting the 5'-untranslated region of the hepatitis A virus detected 0.5 infectious units of HAV and 40 copies of a synthetic transcript.