What question did this study set out to answer?

The aim is to optimize the extraction of total phenolic content and antioxidant capacity from Orthosiphon aristatus leaves.

Response surface-based optimization of antioxidant-rich extracts from Orthosiphon aristatus (Blume) Miq. via microwave-assisted extraction

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Randomized trial optimized antioxidant extraction in Orthosiphon aristatus, indicating effective methods for bioactive compound retrieval.

The aim is to optimize the extraction of total phenolic content and antioxidant capacity from Orthosiphon aristatus leaves.
Utilized microwave-assisted extraction (MAE) combined with response surface methodology (RSM) and Box-Behnken design.
Evaluated the effects of extraction time, ethanol concentration, and solid-liquid ratio on extraction yields.
Antioxidant capacity was assessed using DPPH, ABTS, FRAP, and CUPRAC assays.
Optimal extraction conditions achieved were 3.465 minutes, 60% ethanol concentration, and a 1:20 solid-liquid ratio.
The process resulted in high levels of total phenolic content and antioxidant activities.
The optimization model showed a desirability score of 0.81 with minimal deviation from predicted values.

BOVANI et al. (2026) studied this question.

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Randomized trial optimized antioxidant extraction in Orthosiphon aristatus, indicating effective methods for bioactive compound retrieval.