Randomized trial evaluates antimicrobial efficacy of Calotropis procera stem bark against clinical pathogens, indicating potential for drug development.
Key Points
The study investigates the phytochemical composition and antimicrobial properties of Calotropis procera stem bark extracts against clinical pathogens.
Extracts were prepared using methanol and ethyl acetate for phytochemical screening.
Antimicrobial activity was assessed using agar well diffusion and broth dilution for MIC and MBC/MFC assays.
Column chromatography was used for bioassay-guided fractionation to isolate active compounds.
The methanol extract showed broad-spectrum activity, particularly against MRSA and other pathogens, with MIC values of 0.63 to 1.25 mg/mL.
Isolated compounds SCP-A and SCP-B exhibited stronger antimicrobial effects compared to crude extracts.
SCP-C displayed limited activity against the tested pathogens.