Key result
Sequence analysis of recombinant plasmids from chicken leg muscle revealed two distinct alpha-tropomyosin mRNAs with similar deduced protein sequences but considerable variation in nucleotide sequence.
The study identifies two distinct alpha-tropomyosin mRNA species in chicken skeletal muscle with significant nucleotide sequence variation despite similar protein sequences.
No immediate clinical implications; leaves open whether analogous alpha-tropomyosin variants exist or function in human cardiac muscle.
Recombinant plasmids have been isolated which contain sequences complementary to two distinct alpha-tropomyosin mRNA species present in chicken leg muscle. The proteins coded for by these different mRNAs could be distinguished by their electrophoretic behaviour in the presence of 3.5 M urea. The properties of the minor alpha-tropomyosin of chicken leg muscle were similar to those reported for the alpha-tropomyosin of slow twitch chicken skeletal muscle. Sequence analysis of available plasmids showed that the deduced protein sequences of both types of alpha-tropomyosin were very similar and closely related to the known protein sequence of rabbit alpha-tropomyosin. However considerable variation in nucleotide coding sequence of the two alpha-tropomyosin mRNAs was found.
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Alexander R. MacLeod (1982) studied this question. Sequence analysis of recombinant plasmids from chicken leg muscle revealed two distinct alpha-tropomyosin mRNAs with similar deduced protein sequences but considerable variation in nucleotide sequence.
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