Anti-Inflammatory Activity of Purified 36.3 kDa Coleus Aromatics Leaves Protein: An in Vitro Study

Background and aim: Medicinal plants have recently become the focus of intense study for conservation, as documented pharmacological effects support their traditional uses. This study aims to investigate the anti-inflammatory activity of purified 36.3 kDa Coleus aromaticus leaves protein (CALP) using the Human Red Blood Cells (HRBC) membrane stabilization method. Purified 36.3kDa CALP is used for this study. Material and methods: The HRBC membrane stabilization method was employed to investigate the anti-inflammatory activity of CALP. All data were expressed as mean ± standard deviation of three replicates (n=3). Results: The anti-inflammatory activity of the purified CALP was concentration-dependent; as the concentration increased, the percentage of protection also increased. The purified CALP shows a maximum anti-inflammatory activity of 65.93% (±3.12) at 500 μg/ml, which is slightly lower than that of the standard drug Diclofenac sodium [80.34% (±3.90)] at 200 μg/ml. The purified Coleus aromaticus leaves protein exhibits a membrane stabilization effect by inhibiting hypotonicity-induced lysis of erythrocyte membrane. Conclusions: This study concludes that the purified 36.6 kDa CALP exhibits a stronger membrane-stabilizing property than the standard drug Diclofenac sodium and thus has stronger anti-inflammatory activity. Proteins will continue to serve as a reservoir for the development of potent drugs with less severe and life-threatening adverse effects; however, these results need to be confirmed by in vivo anti-inflammatory studies.