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June 17, 2026Analytical Chemistry

Multifocal Pixel/Photon-Reassignment FLIM (MPPR-FLIM): A Super-Resolution Analytical Tool for Characterizing Subcellular Fluorescence Lifetime Heterogeneity via TCSPC

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Authors

YLYongtu LiaoDLDanying LinDCDuo Chen

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Overview

Randomized trial demonstrates enhanced fluorescence lifetime imaging in live cells, suggesting practical advances in biomedical research.

Key Points

  • This study aims to address spatial resolution limitations in fluorescence lifetime imaging (FLIM) for better characterization of subcellular environments.
  • Developed a super-resolution technique named MPPR-FLIM.
  • Utilized a wide-field and single-point detector along with TCSPC for fluorescence measurements.
  • Implemented a data remapping algorithm to enhance imaging resolution without custom fabrication.
  • Achieved ∼2.1-fold improvement in spatial resolution compared to conventional TCSPC-FLIM systems.
  • Successfully characterized fluorescence lifetime variations in lysosomes of live cells.
  • Demonstrated potential for linking subcellular lifetime heterogeneity with cellular functions.

Cite This Study

Liao et al. (2026) studied this question.

synapsesocial.com/papers/6a323c94d50b63ecad206b09https://doi.org/10.1021/acs.analchem.5c07992
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Pixel super-resolved fluorescence lifetime imaging using deep neural networks2026
  2. 2BPS2026 – Combining ISM and FLIM for high-fidelity lifetime imaging in dense environments2026
  3. 3Research highlight: new and powerful approach to wide-field fluorescence lifetime imaging with single-molecule sensitivity2025
  4. 4Multiplexed lifetime imaging of single molecules with a gated single-photon camera2024 · 1 citations
  5. 5Large-FOV, dual-region, two-photon fluorescence lifetime imaging microscopy (LD-2P-FLIM) for multiparameter and quantitative brain function imaging2026