What question did this study set out to answer?

This research aims to evaluate and compare the accuracy of sputum-based targeted sequencing against isolate-based whole-genome sequencing in predicting drug resistance in tuberculosis.

June 17, 2026International Journal of Molecular SciencesOpen Access

Evaluating the Performance of Sputum-Based Targeted Sequencing Against Mycobacterial Whole-Genome Sequencing in Predicting Tuberculosis Drug Resistance

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Authors

LTLili TianTianjin University NWN H WangBeijing Center for Disease Prevention and Control DXDai XiaoweiBeijing Center for Disease Prevention and Control

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Implication

Randomized trial compares sputum-based targeted sequencing to whole-genome sequencing for drug resistance in tuberculosis, indicating method differences in resistance profiling.

Key Points

This research aims to evaluate and compare the accuracy of sputum-based targeted sequencing against isolate-based whole-genome sequencing in predicting drug resistance in tuberculosis.
Compared sputum-targeted next-generation sequencing (tNGS) and whole-genome sequencing (WGS) in 68 culture-positive tuberculosis specimens.
Used phenotypic drug susceptibility testing (pDST) as a reference for evaluating resistance predictions.
Assessed performance for lineage concordance and sensitivity/specificity between the two methods.
tNGS showed 90.9% sensitivity and 65.2% specificity for rifampicin; WGS had an overall agreement of 86.8%.
tNGS sensitivity for isoniazid was 82.6% while WGS was 78.3%, with WGS having higher specificity (91.1% vs. 75.6%).
For ethambutol, tNGS sensitivity was superior (80% vs. 40%), but both methods performed poorly for pyrazinamide (~40% agreement).

Cite This Study

Tian et al. (2026) studied this question.

synapsesocial.com/papers/6a323cc9d50b63ecad206df6 https://doi.org/10.3390/ijms27125392

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Implication

Randomized trial compares sputum-based targeted sequencing to whole-genome sequencing for drug resistance in tuberculosis, indicating method differences in resistance profiling.

Evaluating the Performance of Sputum-Based Targeted Sequencing Against Mycobacterial Whole-Genome Sequencing in Predicting Tuberculosis Drug Resistance

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Key Points

Cite This Study

Evaluating the Performance of Sputum-Based Targeted Sequencing Against Mycobacterial Whole-Genome Sequencing in Predicting Tuberculosis Drug Resistance

Discussion

Member takes

Implication

Key Points

Cite This Study

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