What question did this study set out to answer?

The aim is to analyze the effect of light on the mRNA rhythms of melanopsins and endothelin receptor in Xenopus laevis melanophores.

June 19, 2026Sleep ScienceOpen Access

mRNA RHYTHMS OF MELANOPSINS AND ENDOTHELIN RECEPTOR IN Xenopus laevis MELANOPHORES AND THEIR MODULATION BY LIGHT

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Authors

MMMNCM MoraesUniversidade de São Paulo ACAML CastrucciUniversidade de São Paulo

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Implication

Quantitative PCR reveals light-regulated expression of melanopsins and endothelin receptor in Xenopus laevis melanophores, indicating their role in pigment response.

Key Points

The aim is to analyze the effect of light on the mRNA rhythms of melanopsins and endothelin receptor in Xenopus laevis melanophores.
Quantitative PCR was performed on melanophores cultured under a 12L:12D light regimen.
Total RNA extraction was conducted every four hours starting from ZT0 on the sixth day for analysis.
Gene expression of Opn4m, Opn4x, and ETc was quantified.
Opn4x mRNA expression increased during the light phase, peaking at ZT0.
A non-significant trend was observed for Opn4m mRNA during the dark phase.
ETc mRNA showed no temporal variation throughout the 24-hour period.

Cite This Study

Moraes et al. (2009) studied this question.

synapsesocial.com/papers/6a34dbc465a5b0777af2c657 https://doi.org/10.1055/s-0046-1820623

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Implication

Quantitative PCR reveals light-regulated expression of melanopsins and endothelin receptor in Xenopus laevis melanophores, indicating their role in pigment response.

mRNA RHYTHMS OF MELANOPSINS AND ENDOTHELIN RECEPTOR IN Xenopus laevis MELANOPHORES AND THEIR MODULATION BY LIGHT

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Cite This Study

mRNA RHYTHMS OF MELANOPSINS AND ENDOTHELIN RECEPTOR IN Xenopus laevis MELANOPHORES AND THEIR MODULATION BY LIGHT

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