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This research aims to explore the modulation of mRNA and protein rhythms in chicken embryonic retinal cells by light and glutamate.

June 19, 2026Sleep ScienceOpen Access

PROTEIN AND mRNA RHYTHMS IN CHICKEN EMBRYONIC RETINAL CELLS AND THEIR MODULATION BY LIGHT AND GLUTAMATE

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Randomized trial investigates mRNA and protein rhythms in embryonic retinal cells, highlighting glutamate's role in circadian modulation.

This research aims to explore the modulation of mRNA and protein rhythms in chicken embryonic retinal cells by light and glutamate.
Isolated primary cultures of chicken retina from 8-day-old embryos were kept in constant dark or 12L:12D photoperiod for 5 days.
Experiment included treatment with 100uM glutamate for 12 hours starting at ZT0 of the fifth day in vitro.
Total RNA extraction and quantitative PCR were performed every three hours for mRNA analysis, alongside immunocytochemistry and Western blot for protein assessment.
No rhythm of transcription was observed in constant dark conditions for any gene analyzed.
Under 12L:12D photoperiod, all genes exhibited phase variations in expression.
Light and glutamate modulate mRNA transcription and protein synthesis in embryonic retinal cells.

Lima et al. (2009) studied this question.

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