Key result
GAstV-2 exhibits broad tissue tropism and primarily targets renal tubular epithelial cells.
Why the study?
Goose astrovirus 2 causes visceral gout in goslings, but its distribution and localization in different tissues over time required further investigation.
GAstV-2 has a broad tissue tropism in goslings and primarily targets the kidneys, providing a basis for understanding the pathogenesis of GAstV-2-induced visceral gout.
mAb-based IHC reveals early widespread GAstV-2 antigen distribution in gosling tissues; leaves open optimal diagnostics and interventions for industry losses.
Goose astrovirus 2 (GAstV-2) causes visceral gout in goslings and has resulted in significant economic losses in the goose industry of China since its outbreak in 2017. To further investigate the distribution and localization of GAstV-2 in different tissues at different times, a monoclonal antibody (mAb)-based immunohistochemical (IHC) assay was developed to detect GAstV-2. A total of 80 1-day-old healthy goslings were inoculated with GAstV-2 via the oral (n = 40) and intramuscular routes (n = 40). GAstV-2 in the tissues of interest was detected using the established IHC assay. The results showed that positive signals were detected in most tissues at 1 day post-infection (dpi). Viral antigens were mainly distributed in the cytoplasm, and the staining intensity was higher in the renal tubular epithelial cells than in other cells. Taken together, our data demonstrated that GAstV-2 has a broad tissue tropism and primarily targets the kidneys. These results are likely to provide a scientific basis for further elucidation of the pathogenesis of GAstV-2.
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Wei et al. (2023) studied Goose astrovirus 2 (GAstV-2) infection (n=120). GAstV-2 inoculation vs. Sterile PBS was evaluated on Localization and distribution of GAstV-2 antigens in tissues. GAstV-2 has a broad tissue tropism and primarily targets the kidneys, with viral antigens mainly distributed in the cytoplasm and highest staining intensity in renal tubular epithelial cells.
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