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June 20, 2026Biomass and BioenergyOpen Access

Integrating high solids enzymatic hydrolysis and co-culture fermentation to improve ethanol production from deep eutectic solvent pretreated rice straw

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Authors

LILonginus Ifeanyi IgbojionuYMYujie MaoEBEleanor Binner

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Overview

Randomized trial integrates enzymatic hydrolysis and co-culture fermentation to enhance ethanol yield from rice straw, suggesting a sustainable approach to bioethanol production.

Key Points

  • To develop an integrated process that enhances ethanol production from rice straw by addressing major bottlenecks in biomass conversion.
  • Microwave-assisted deep eutectic solvent pretreatment of rice straw.
  • Optimized high-solids enzymatic hydrolysis with central composite design.
  • Co-culture fermentation using Saccharomyces cerevisiae and Candida tropicalis.
  • Achieved a total sugar yield of 94.92% at 9.89% solids loading.
  • Co-culture fermentation resulted in a maximum ethanol concentration of 41.1 g/L with a yield of 0.46 g/g and productivity of 1.71 g/L.h.
  • Lower yields (0.35 g/g) and productivity (0.67 g/L.h) were observed with hydrolysates from 1% H2SO4 pretreatment.

Cite This Study

Igbojionu et al. (2026) studied this question.

synapsesocial.com/papers/6a362ee4db0793dc1a53674fhttps://doi.org/10.1016/j.biombioe.2026.109712
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