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September 5, 2026Industrial Crops and ProductsOpen Access

Bridging industrial pretreatment and downstream bioconversion through low-enzyme high-solids ethanol production from unwashed wheat straw

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Authors

YFYuyang FanRWRou WengYGYuanjia Gu

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Overview

Experimental study demonstrates efficient ethanol production from unwashed wheat straw using low enzyme loadings and fed-batch feeding, indicating a cost-effective pathway for biofuel processing.

Key Points

  • To develop a low-water and low-enzyme fed-batch strategy for producing ethanol from unwashed industrial wheat straw pretreated by mechanical extrusion and steam explosion.
  • Evaluated enzymatic hydrolysis under solids loadings up to 30% (w/w) and enzyme dosages ranging from 2.7 to 10 FPU/g dry substrate.
  • Analyzed slurry rheology, water mobility, and component removal to characterize physical and biomass-derived mass-transfer constraints.
  • Implemented alkaline neutralization, poly(vinyl alcohol) enzyme protection, and fed-batch substrate feeding on washed and unwashed pretreated biomass.
  • Combining sodium hydroxide neutralization, poly(vinyl alcohol) enzyme protection, and fed-batch feeding increased hydrolysis yield by 18.4%.
  • Washed pretreated wheat straw reached a maximum ethanol titer of 71.4 g/L at 30% solids loading and 10 FPU/g dry substrate.
  • The optimized unwashed fed-batch process achieved 35.4 g/L ethanol at 25% solids loading using 2.7 FPU/g dry substrate, resulting in a hydrolysis-stage consumables cost of 2988.8 CNY per metric tonne of ethanol.

Cite This Study

Fan et al. (2026) studied this question.

synapsesocial.com/papers/6a9bd4216b95aff0620eb834https://doi.org/10.1016/j.indcrop.2026.124311
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