Human Recombinant Interleukin-3 (hrIL-3) Stimulates FSHR and LHCGR Expression in Ovaries of Female Rats

To investigate the impact of human recombinant interleukin-3 (hrIL-3) on ovulation in female rats ovaries. We used forty eight Wister rats were divided randomly into four equal groups twelve female each. All rats undergone superovulation protocols using pregnant mare serum gonadotropin (PMSG) 10IU intra-peritoneally. Final maturation of oocytes achieved after 48hours using human chorionic gonadotropin (hCG) 10IU intra-peritoneally. We used hrIL-3 in three doses: 15ng, 30ng, and 45ng/rat after superovulation. Concomitantly with hCG, intravenous injection of hrIL-3 was used in three doses o the three treated groups, while the control group received normal saline at the same time of hCG injection. The animals of each group were divided into two subgroups six female each According to the time of sacrificing after 12h and 36h of hCG injection. Samples of ovaries were obtainedv from all animals for RT-qPCR gene expression of fshr and lhcgr. There was a significant increase (P<0.05) of fshr gene after 12h of treatment in 15ng and 45ng hrIL-3 compared with control. However, there were no significant differences among the other groups compared to control. There is significant increase (P<0.05) of lhcgr gene after 12h of treatment in 15ng hrIL-3 compared with control, while there were no significant differences among the other groups. After 36h, lhcgr gene showed significant increase (P<0.05) of 45ng compared with control while there were no significant differences among the other groups.hrIL-3 with a dose of 45ng at 36h after hCG injection stimulates rat ovaries via increase gene expression of FSHR and LHCGR in ovarian tissues.