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To explore the effects of human PMP22 expression on myelination in Schwann cells and the associated mechanisms causing dysmyelination.

Stable and Tunable Expression of Human Peripheral Myelin Protein 22 in Rat Schwann Cells

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To explore the effects of human PMP22 expression on myelination in Schwann cells and the associated mechanisms causing dysmyelination.
Developed a clonal rat Schwann cell line for myc-tagged human PMP22 expression.
Tuned PMP22 expression levels through doxycycline concentration in culture media.
Analyzed localization and retention of PMP22 protein under varying expression levels.
Maximal mycPMP22 expression achieved at 800–1600 ng/mL.
High levels of mycPMP22 resulted in increased intracellular retention and formation of puncta.
Percentage of mycPMP22 at the plasma membrane inversely correlates with expression level.

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Randomized trial investigates PMP22 protein effects on myelin in Schwann cells, suggesting mechanisms for CMT diseases.

Wilkinson et al. (2026) studied this question.

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