Multicenter Analytical Comparison of Automated Quantitative Assays Versus Line Immunoassay for Anti‐Ro/La Antibody Detection in Primary Sjögren’s Syndrome
Multicenter analytical comparison of automated assays for anti-Ro/La detection in patients with primary Sjögren’s syndrome, indicating high specificity and accuracy.
Key Points
This study aimed to compare automated quantitative assays with line immunoassay for detecting anti-Ro/La antibodies in primary Sjögren’s syndrome.
Analyzed serum samples from 434 patients with primary Sjögren’s syndrome and 100 healthy controls using LIA, CLIA, and MBI.
Assessed sensitivity, specificity, and qualitative agreement among assays.
Conducted ROC analysis and DeLong test for comparing analytical accuracy.
LIA demonstrated the highest sensitivity for anti-Ro60 (92.6%) and anti-Ro52 (89.2%).
CLIA and MBI showed comparable sensitivity for anti-La (50.9% vs. 50.2% vs. 48.2%).
MBI achieved the highest AUC values for anti-Ro60 (0.980), anti-Ro52 (0.970), and anti-La (0.935), surpassing both CLIA and LIA (p < 0.05).