Key result
Chronic exposure of cultured embryonic phasic smooth muscle cells to endothelin-1 prolonged time to peak force and reduced peak force, converting them to a tonic contractile phenotype.
Population
Cultured embryonic smooth muscle cells (gizzard/phasic and aortic/tonic) from chick embryos
Comparison
Chronic exposure to endothelin-1 vs Unexposed cultured embryonic gizzard cells
Design
Preclinical
Authors
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Endothelin-1 may specify embryonic smooth muscle phenotypes; hypothesis-generating in vitro result leaves open in vivo and clinical relevance.
Endothelin-1 can convert phasic smooth muscle cells to a tonic contractile phenotype in vitro, suggesting a developmental role in specifying smooth muscle phenotypes.
Fisher et al. (1997) studied this question. Endothelin-1 vs. Untreated phasic (gizzard) cells was evaluated on Contractile properties (time to peak force and peak force). Chronic exposure of cultured embryonic phasic smooth muscle cells to endothelin-1 prolonged time to peak force and reduced peak force, converting them to a tonic contractile phenotype.
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