Key result
Deletion of the second translation initiation site of foot-and-mouth disease virus abolished virus replication in transfected BHK cells, whereas deletion of the first site did not.
The second translation initiation site of foot-and-mouth disease virus is essential for virus replication, whereas the first site is dispensable.
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May enable FMDV attenuation strategies; leaves open in vivo validation and therapeutic translation.
The effect of deletion of each of the two authentic polyprotein translation initiation sites of foot-and-mouth disease virus on viral protein synthesis and replication was analyzed. Deletion of either the first or the second initiation site led to the expression of only one form of the leader protein, L or L', respectively, but in vitro processing of the viral polyprotein and cleavage of eIF-4 gamma were not affected by either deletion. Whereas RNA in which the first translation initiation site had been deleted led to the production of viruses in transfected BHK cells, deletion of the second translation initiation site abolished virus replication.
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Cao et al. (1995) studied Foot-and-mouth disease virus. Deletion of translation initiation sites was evaluated on Viral protein synthesis and replication. Deletion of the second translation initiation site of foot-and-mouth disease virus abolished virus replication in transfected BHK cells, whereas deletion of the first site did not.
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