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June 29, 2026Scientific ReportsOpen Access

Isolation, screening, and molecular characterization of indigenous yeast strains from fruits for bioethanol production using molasses

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Authors

TJTamene Milkessa JiruMMMarta Mamo

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Overview

Randomized trial isolates and characterizes yeast strains for bioethanol production from fruits, suggesting renewable energy sources.

Key Points

  • The aim was to isolate and characterize indigenous yeast strains from fruits for bioethanol production using molasses.
  • Eighteen fruit samples were collected from Gondar City, Ethiopia.
  • Yeasts were isolated using serial dilution and screened based on gas production and color change.
  • Molecular identification involved sequencing ITS1, ITS2, and 5.8 S rRNA regions.
  • Four yeast isolates showed strong fermentation potential with ethanol concentrations of 0.780 to 1.218%.
  • Fermentation efficiencies ranged from 36.0% to 47.0%, and productivity was between 0.16 to 0.23 g/L/h.
  • A1IS3 was identified as Pichia kudriavzevii and AS2M2 as Pichia species.

Cite This Study

Jiru et al. (2026) studied this question.

synapsesocial.com/papers/6a420ab2f91bb43ea9191fd4https://doi.org/10.1038/s41598-026-57259-x
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