Key result
Entelon150 significantly reduced the calcium content of implanted bovine pericardium tissue compared to saline control (0.56 vs 1.48 mg/g) in a beagle dog model.
Why the study?
Inflammation and calcification cause bioprosthetic valve degeneration, prompting evaluation of the anti-inflammatory and anti-calcification effects of grape-seed extract in an animal model.
Does vitis vinifera seed extract reduce inflammation and calcification of implanted bovine pericardium in a beagle dog model?
Does vitis vinifera seed extract reduce inflammation and calcification of implanted bovine pericardium in a beagle dog model?
Vitis vinifera seed extract significantly attenuates post-implantation inflammation and degenerative calcification of bovine pericardium in a dog model, suggesting potential to increase bioprosthetic valve longevity.
BACKGROUND AND AIMS: Inflammation and calcification are major factors responsible for degeneration of bioprosthetic valve and other substitute heart valve implantations. The objective of this study was to evaluate the anti-inflammatory and anti-calcification effects of Entelon150® (consisting of grape-seed extract) in a beagle dog model of intravascular bovine pericardium implantation. METHODS: In total, 8 healthy male beagle dogs were implanted with a bovine pericardium bilaterally in the external jugular veins and divided into two groups. Animals in the Entelon150® group (n = 4) were treated with 150 mg of Entelon150® twice daily for six weeks after surgery. The negative control (NC) group (n = 4) was treated with 5 ml of saline using the same method. After six weeks, we measured the calcium content, performed histological examination, and performed molecular analysis. RESULTS: The calcium content of implanted tissue in the Entelon150® group (0.56±0.14 mg/g) was significantly lower than that in the NC group (1.48±0.57 mg/g) (p < 0.05). Histopathological examination showed that infiltration of chronic inflammatory cells, such as fibroblasts and macrophages, occurred around the graft in all groups; however, the inflammation level of the implanted tissue in the Entelon150® group was s lower than that in the NC group. Both immunohistochemical and western blot analyses revealed that bone morphogenetic protein 2 expression was significantly attenuated in the Entelon150® group. CONCLUSIONS: Our results indicate that Entelon150® significantly attenuates post-implantation inflammation and degenerative calcification of the bovine pericardium in dogs. Therefore, Entelon150® may increase the longevity of the bovine pericardium after intravascular implantation.
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Choi et al. (2021) studied Bioprosthetic valve calcification (n=8). Entelon150 (grape-seed extract) vs. Saline (5 ml twice daily) was evaluated on Calcium content of implanted tissue (mg/g) (p=<0.05). Entelon150 significantly reduced the calcium content of implanted bovine pericardium tissue compared to saline control (0.56 vs 1.48 mg/g) in a beagle dog model.
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