Peripheral T cell receptor beta immune repertoire is promptly reconstituted after acute myocardial infarction

Figures

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  Fig. 1. AMI leads to αβ T cells activation. Peripheral blood samples were obtained from 30 healthy controls and 30 AMI patients. a The count of WBC was measured from peripheral blood samples. The percentage of neutrophils (b), lymphocytes (c), monocytes (e) was detected by Sysmex XN in our clinical laboratory. d The correlation between cTnI level and lymphocytes was calculated in AMI patients. Spearman’s correlation coefficient r = − 0.5874, P = 0.0006 (n = 30). f The percentage of αβ T cells from peripheral blood was analyzed by flow cytometry. g αβ T cells were gated and tested for expression of CD69

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  Fig. 2. The usage patterns of V and J gene segments after AMI. High-throughput sequencing of 10 PCR products (five controls and five AMI patients) amplified from peripheral lymphocytes for TCRβ repertoire analysis. The distribution of Top10V (a) and Top5J (b) genes expression. Colors indicate Top10/Top5 clones (each color represents a clone); grays represent non-Top10/Top5 clones. c Heatmaps of hierarchical clustering of V and J gene segments frequencies. The frequencies of V (d, f) and J (e, g) gene segments usage that had significant differences between healthy controls and AMI patients

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  Fig. 3. The usage patterns of V–J and V–D–J combination. a Circular plots representing TCRβ loci were identified from αβ T cells. The clonotypes of V–J (b) and V–D–J (c) combinations were counted from sequencing data. d Volcano plots showing the comparison of V–J and V-D–J combinations between healthy controls and AMI patients (red dots refer to the differentially expressed combinations with statistical significance)

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  Fig. 4. The diversity of TCRβ CDR3 AA clonotypes. a Quantification (plot grays) and frequencies (plot colors) of overlapping TCRβ clonotypes. Light grays indicate the overlapping clonotypes, while dark grays indicate the total clonotypes per sample. Comparison of TCRβ repertoire diversity by total CDR3 AA clonotypes (b), shared CDR3 AA clonotypes (c), Gini coefficient (d), Simpson index (e) and Shannon index (f) between healthy controls and AMI patients. g Rank-Abundance analysis of CDR3 AA clonotypes among different samples

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  Fig. 5. Reconstitution of CDR3 AA after AMI. a Bhattacharyya distance analysis for the similarity of CDR3 AA from different group. b Comparison of the fraction of Top20 CDR3 AAs between healthy controls and AMI patients. c The frequencies of CDR3 AA that had significant differences were shown