Comprehensive analysis of T-cell receptor repertoire in patients with acute coronary syndrome by high-throughput sequencing

Figures

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  Fig. 1. Clonal distribution of T cells in patients with UA, AMI and NCA. a Frequency of unique TCR β sequences identified in the peripheral blood of patients with UA, AMI and NCA. Differences between groups were compared using one-way ANOVA. b Percentage frequency of top 200 TCRβ nucleotide sequences in UA, AMI and NCA. Differences between groups were compared using one-way ANOVA. c Frequency distributions of TCRβ CDR3 clones from UA(n = 9), AMI(n = 14) and NCA(n = 9) patients. Differences between groups were compared using one-way ANOVA. d Diversity metrics for TCRβ CDR3 repertoires in UA, AMI and NCA. Data were compared using Wilcoxon rank-sum test. *P < 0.05, **P < 0.01, ***P < 0.001

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  Fig. 2. Heatmap of Vβ and Jβ gene segments usage in samples from UA, AMI and NCA. The heatmap bar indicates the usage frequency of Vβ or Jβ gene segments in each sample

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  Fig. 3. Vβ and J β gene usage of clonotypes in patients with UA, AMI and NCA. The frequencies of Vβ and Jβ genes were calculated and compared between AMI, UA and NCA. a Vβ genes with different frequencies between AMI and NCA; b Vβ genes with different frequencies between UA and NCA; c Vβ genes with different frequencies between AMI and UA; d Jβ gene with different frequencies between AMI, UA and NCA. Data were compared using Wilcoxon rank-sum test. *P < 0.05, **P < 0.01, ***P < 0.001

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  Fig. 4. Overlap in T-cell specificity in each group and between disease groups. a Data show the overlap of clonotypes in each disease group, UA, AMI and NCA. b Data show the overlap of clonotypes between disease groups, UA and AMI, UA and NCA, AMI and NCA. Data were compared using Wilcoxon rank-sum test. *P < 0.05, **P < 0.01, ***P < 0.001