ObjectiveTo investigate the ameliorative effects of live-combined Bifidobacterium, Lactobacillus, and Enterococcus (LCBLE) on heat tolerance and systemic inflammatory injury in rats with heat stroke, and to explore its underlying mechanisms.MethodsRats were randomly divided into blank control, LCBLE control, model control, and LCBLE treatment groups. The LCBLE-treated groups received daily intragastric administration of LCBLE for 7 consecutive days, while the control groups received equal volumes of normal saline. On day 8, heat stroke was induced in the model control and LCBLE treatment groups. Core body temperature, body weight, and survival rate were monitored. Serum inflammatory cytokines were measured by enzyme-linked immunosorbent assay, and intestinal barrier function was assessed by blood biomarkers. Tight junction proteins zonula occludens-1 (ZO-1) and occludin were localized by immunofluorescence, intestinal ultrastructure was observed by transmission electron microscopy, and pathological morphology was evaluated by hematoxylin-eosin staining. Gut microbial composition was analyzed by 16S rRNA sequencing.ResultsLCBLE pretreatment significantly reduced peak core body temperature [(42.03±0.43) ℃ vs. (43.21±0.33) ℃, Pvs. (286.7±12.6) g, Pvs. 55.6%, PPPPBifidobacterium and Lactobacillus in the gut.ConclusionPretreatment with LCBLE improves heat tolerance and alleviates systemic inflammation in heat-stroke rats, likely through regulating gut microbiota and maintaining intestinal barrier integrity.
XIE et al. (2026) studied this question.