What is the clinical evidence from this study?

Study design: Cohort. Population: Bleeding disorder (n=150). Intervention: Multiple platelet dense granule ATP release tests. Primary outcome: Consistency of ATP release findings between first and second tests.

What question did this study set out to answer?

Investigate variability in platelet dense granule ATP release findings among patients assessed multiple times.

August 30, 2016International Journal of Laboratory Hematology

Variability in platelet dense granule adenosine triphosphate release findings amongst patients tested multiple times as part of an assessment for a bleeding disorder

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Key result

Platelet dense granule ATP release testing showed inconsistent findings in 36-39% of subjects tested multiple times, suggesting limited value for diagnosing platelet disorders.

Why the study?

Is lumi-aggregometry quantification of platelet dense granule ATP release a reliable diagnostic test for platelet function disorders?

Population

150 unique subjects assessed for dense granule ATP release defects more than once

Design

Cohort

Authors

MBMatthew BadinAstraZeneca (Canada)LGLukas GrafUniversity Children's Hospital Zurich JIJanaki Krishnamoorthy IyerNational University of Singapore

Discussion

Member takes

Implication

High intra-patient variability cautions against relying on single ATP release tests for diagnosis; leaves open need for validated protocols or alternatives.

Key Points

Investigate variability in platelet dense granule ATP release findings among patients assessed multiple times.
Analyzed data from first and second ATP release tests for multiple subjects between January 2007 and June 2013 (cohort I).
Conducted similar analyses for subjects in a platelet disorder study assessed before October 2015 (cohort II).
Normal ATP release confirmed in 83% of subjects in cohort I and 100% in cohort II.
Impaired ATP release with multiple agonists confirmed in 34% (cohort I) and 54% (cohort II) of subjects.
Inconsistent findings were noted in 36% (cohort I) and 39% (cohort II) of cases.

Study Design

Type

Cohort (n=150)

Structured PICO

Is lumi-aggregometry quantification of platelet dense granule ATP release a reliable diagnostic test for platelet function disorders?

Population

150 subjects assessed for dense granule ATP release defects more than once to evaluate test variability.

Exposure

Lumi-aggregometry quantification of platelet dense granule ATP release (repeated testing)

Outcome

Consistency of platelet dense granule ATP release findings between first and second testssurrogate

Lumi-aggregometry for platelet dense granule ATP release shows significant intra-patient variability, limiting its diagnostic value for platelet disorders.

Cite This Study

Badin et al. (2016) conducted a cohort in Bleeding disorder (n=150). Multiple platelet dense granule ATP release tests was evaluated on Consistency of ATP release findings between first and second tests. Platelet dense granule ATP release testing showed inconsistent findings in 36-39% of subjects tested multiple times, suggesting limited value for diagnosing platelet disorders.

synapsesocial.com/papers/6a4af0d14e2f04c47c814504 https://doi.org/10.1111/ijlh.12553

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Implication

High intra-patient variability cautions against relying on single ATP release tests for diagnosis; leaves open need for validated protocols or alternatives.

Variability in platelet dense granule adenosine triphosphate release findings amongst patients tested multiple times as part of an assessment for a bleeding disorder

Discussion

Member takes

Implication

Key Points

Study Design

Structured PICO

Cite This Study

Variability in platelet dense granule adenosine triphosphate release findings amongst patients tested multiple times as part of an assessment for a bleeding disorder

Discussion

Member takes

Implication

Key Points

Study Design

Structured PICO

Cite This Study

Also Consider

Also Consider