In this study, we have a type of functional nanofibers, hybrid nanofibers containing Polycaprolactone (PCL) / Chitosan (CS) and Polyvinyl alcohol (PVA) were successfully fabricated by hybrid electrospinning method. Pioglitazone Hydrochloride (PIO) was incorporated in PCL/CS solution due to its hydrophobic nature, and Gemcitabine Hydrochloride (GEM) was incorporated in PVA solution due to its hydrophilic nature. The prepared hybrid PCL-CS-PIO/PVA-GEM nanofibers exhibited a biphasic release profile in each case, indicating an initial burst followed by a sustained phase of GEM and PIO over 5 days. The maximum amount released from the hybrid nanofibers reached 75.88 ± 1.5% of the GEM and 64.54 ± 2.05% of the PIO in a medium of pH 7.4. Flow cytometry and MTT analyses demonstrated that the combination of PIO and GEM induced apoptosis and inhibited the growth of melanoma cancer cell lines by inhibiting proliferation with cell viability of 51.34 ± 2.2% and 46.04 ± 1.5% after 24 and 48 h compared to control group. Obtained nanofibers were assessed for their chemical composition and morphology followed by scanning electron microscopy (SEM), FTIR, contact angle measurements and drug release profile. Then, a cytotoxicity study was performed on human melanoma cancer cell line, followed by investigating its potential in treatment applications.
Jafari-Najaf-Abadi et al. (Sat,) studied this question.