Elevated circulating HSP70 levels in AMI patients correlated with monocyte TLR4 and inflammatory cytokines, and were higher at 14 days in patients developing heart failure (n=15).
Observational (n=72)
Are elevated circulating levels of HSP70 associated with systemic inflammation and heart failure progression after acute myocardial infarction?
Elevated circulating HSP70 levels after AMI correlate with TLR4-mediated systemic inflammation and the development of heart failure.
BACKGROUND: Recent studies have shown that heat shock protein (HSP) 70 may serve as a "damage signal" to the immune system and could be the endogenous ligand for Toll-like receptor (TLR) 4 mediating synthesis of inflammatory cytokines. AIMS: To explore the relationship between circulating HSP70 levels and activation of monocyte TLR4 and myocardial damage after AMI. METHODS AND RESULTS: This study examined circulating HSP70 and monocyte TLR4 levels in 52 patients with AMI and 20 controls, and analyzed ex vivo inflammatory cytokine productions using HSP70-stimulated monocytes. Circulating HSP70 levels were higher in AMI patients on day 1 after onset than in controls and remained elevated in AMI patients 14 days after onset. HSP70 levels were positively correlated with monocyte TLR4, plasma interleukin-6 and tumor necrosis factor-alpha levels in AMI patients. HSP70 levels 14 days after onset were higher in AMI patients with heart failure (n=15) than in those without heart failure. In our in vitro study, HSP70-stimulated monocytes resulted in dose-dependent TLR4 expression and release of inflammatory cytokines. TLR4 antibody inhibited inflammatory cytokines release. CONCLUSIONS: Elevated circulating levels of HSP70 may be involved in TLR4 signal-mediated immune response and the progression of heart failure after AMI.
Satoh et al. (Tue,) conducted a observational in Acute Myocardial Infarction (n=72). Elevated circulating HSP70 levels vs. Controls was evaluated on Circulating HSP70 and monocyte TLR4 levels, and inflammatory cytokine production. Elevated circulating HSP70 levels in AMI patients correlated with monocyte TLR4 and inflammatory cytokines, and were higher at 14 days in patients developing heart failure (n=15).