Niacinamide/ Co-enzyme Q10 loaded transferosomal gel for anti-aging and enhanced biophysical skin outcome: a split-face clinical study

This study investigated the dermatological outcomes of a transferosomal gel encapsulating Niacinamide and Co-enzyme Q10 compared with a conventional base gel in healthy female volunteers over a 12-week period, with a particular focus on key biophysical and aesthetic skin parameters. Before human use, both formulations underwent primary skin irritation testing on rabbit skin, followed by a patch test on healthy volunteers to establish safety. A randomized split-face study design was implemented. Skin hydration, elasticity, melanin, erythema, sebum content, pore size, spots, and wrinkle indices were systematically assessed. Sensory evaluation and appropriate statistical analyses complemented the biophysical measurements. Irritation and patch-test assessments confirmed the nonirritant, well-tolerated nature of both formulations. The transferosomal gel demonstrated significantly higher efficacy (p < .05) across multiple parameters, including hydration, elasticity, melanin, erythema reduction, and sebum regulation. Improvements in pore size, spot reduction, and wrinkle were also more substantial and became evident as early as week 2, with continual enhancement observed throughout the 12-week study. Transferosomal delivery of Niacinamide and Co-enzyme Q10 demonstrates improved topical efficacy compared with conventional formulation, as evidenced by the performance outcomes. It offers a safe, noninvasive delivery strategy for improving skin-related parameters within the study conditions.