What is the clinical evidence from this study?

Population: Atherosclerosis (n=26). Intervention: Mmp14-/- bone marrow cells vs. Mmp14+/+ bone marrow cells. Primary outcome: Interstitial collagen content in plaques (p=<0.05).

What does this research mean for the field?

MMP-14 deficiency in bone marrow-derived cells promotes interstitial collagen accumulation in atherosclerotic plaques by reducing collagenase activity and pro-MMP-13 activation. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

This research investigates how the absence of MMP-14 in bone marrow-derived cells affects collagen levels in atherosclerotic plaques.

July 10, 2026CirculationOpen Access

Matrix Metalloproteinase-14 Deficiency in Bone Marrow–Derived Cells Promotes Collagen Accumulation in Mouse Atherosclerotic Plaques

Q: What are the key findings of this study?

Engraftment with Mmp14-/- bone marrow resulted in significantly more interstitial collagen in atherosclerotic plaques compared to Mmp14+/+ bone marrow after 16 weeks of an atherogenic diet (P<0.05).

Q: What does this research mean for the field?

MMP-14 deficiency in bone marrow-derived cells promotes interstitial collagen accumulation in atherosclerotic plaques by reducing collagenase activity and pro-MMP-13 activation. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

Q: What question did this study set out to answer?

This research investigates how the absence of MMP-14 in bone marrow-derived cells affects collagen levels in atherosclerotic plaques.

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Key result

Mmp14-/- bone marrow engraftment increases interstitial collagen in atherosclerotic plaques vs Mmp14+/+ marrow.

P<0.05
n=26

Why the study?

Although in vitro results suggest collagenase activity for MMP-14, in vivo evidence for its function in atherosclerosis remained scant.

Does MMP-14 deficiency in bone marrow-derived cells increase collagen accumulation in mouse atherosclerotic plaques?

Key Points

This research investigates how the absence of MMP-14 in bone marrow-derived cells affects collagen levels in atherosclerotic plaques.
Used lethally irradiated low-density lipoprotein receptor-deficient mice reconstituted with bone marrow from Mmp14-/- or Mmp14+/+ mice.
Conducted histological analyses and in vitro collagenase activity assessments after 8 and 16 weeks of atherogenic diet.
Performed Western blot analysis and gelatin zymography on aortic extracts.
Plaques from Mmp14-/- engrafted mice contained significantly more interstitial collagen than those from Mmp14+/+ mice (P<0.05).
Bone marrow-derived macrophages from Mmp14-/- showed significantly less collagenase activity (P<0.01) and stimulated activity (P<0.05).
MMP-14 deficiency resulted in decreased activation of pro-MMP-13 without affecting pro-MMP-2 or pro-MMP-8.

Structured PICO

Does MMP-14 deficiency in bone marrow-derived cells increase collagen accumulation in mouse atherosclerotic plaques?

Population

26 lethally irradiated low-density lipoprotein receptor-deficient mice reconstituted with syngeneic bone marrow cells of Mmp14-/- or Mmp14+/+ mice, fed an atherogenic diet for up to 16 weeks.

Intervention

Engraftment with Mmp14-/- bone marrow cells followed by an atherogenic diet for 8 or 16 weeks

Comparator

Engraftment with Mmp14+/+ bone marrow cells followed by an atherogenic diet for 8 or 16 weeks

Outcome

Interstitial collagen content in aortic root plaquessurrogate

Main Result

p-value: p=<0.05

MMP-14 deficiency in bone marrow-derived cells promotes collagen accumulation in atherosclerotic plaques, highlighting its role in plaque stability.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

May caution against MMP-14 inhibition in atherosclerosis; leaves open its role in human plaque stability.

Cite This Study

Schneider et al. (2008) studied Atherosclerosis (n=26). Mmp14-/- bone marrow cells vs. Mmp14+/+ bone marrow cells was evaluated on Interstitial collagen content in plaques (p=<0.05). Engraftment with Mmp14-/- bone marrow resulted in significantly more interstitial collagen in atherosclerotic plaques compared to Mmp14+/+ bone marrow after 16 weeks of an atherogenic diet (P<0.05).

synapsesocial.com/papers/6a5064af330ff8da2846ea6d https://doi.org/10.1161/circulationaha.107.707448

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Matrix Metalloproteinase-14 Deficiency in Bone Marrow–Derived Cells Promotes Collagen Accumulation in Mouse Atherosclerotic Plaques

Key Points

Structured PICO

Main Result

Discussion

Implication

Cite This Study

Matrix Metalloproteinase-14 Deficiency in Bone Marrow–Derived Cells Promotes Collagen Accumulation in Mouse Atherosclerotic Plaques

Key Points

Structured PICO

Main Result

Discussion

Implication

Cite This Study

Also Consider

Also Consider