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July 10, 2026Reproduction and FertilityOpen Access

In Vitro Mouse Preantral Follicle Development in 2D and Suspension Culture: α-MEM vs. SAGE 1-Step

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Authors

ABAmir BazgirNKNarges KaramiFHFatemeh Hassani

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Overview

Randomized trial explores media effects on oocyte maturation in mouse follicles, suggesting improved fertility outcomes.

Key Points

  • This study aims to investigate how different culture media affect the development and maturation of preantral mouse follicles.
  • Preantral follicles from 14-day-old NMRI mice were cultured for 13 days in either α-MEM or SAGE 1-Step media under 2D or suspension systems.
  • Follicular growth, antrum formation, and gene expression related to maturation and apoptosis were evaluated.
  • HCG was added on day 13 to induce meiotic resumption.
  • SAGE 1-Step significantly improved oocyte maturation (P=0.01 in 2D, P=0.006 in suspension compared to α-MEM).
  • Suspension culture enhanced the percentage of oocytes with normal meiotic spindle organization (P=0.002).
  • The apoptotic ratio of Bax/Bcl2 was significantly decreased in suspension culture (P=0.01).

Cite This Study

Bazgir et al. (2026) studied this question.

synapsesocial.com/papers/6a508ea56eeac72a437a168bhttps://doi.org/10.1530/raf-25-0158
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