Key result
Dark-Pi system enables low-cost, label-free detection of platelet aggregation via light scattering.
Why the study?
Variability in platelet function is not fully understood, and rapid, high-performance hematological tests are needed alongside conventional laboratory measurements for patient stratification and research into antiplatelet drugs.
The Dark-Pi system provides a low-cost, open-source, label-free microfluidic method for monitoring platelet aggregation, which could facilitate large-scale point-of-care platelet function testing.
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May enable accessible platelet testing; leaves open human validation before clinical use.
Measuring platelet function is important for patient stratification to judge bleeding vs. thrombotic risk and for research into antiplatelet drugs to prevent cardiovascular disease. Variability in platelet function is not fully understood, and large studies of inter-individual variation are making gradual progress using laboratory measurements, but rapid and high-performance hematological tests are also needed. We present here a novel microfluidic technology for platelet function analysis that images light scatter by platelets, using a low-cost, open-source, high-throughput and customizable darkfield imaging system called Dark-Pi. The hardware consists of a camera and a simple LED light source controlled by a Raspberry Pi, with 3D-printed parts. Using the Dark-Pi, platelet aggregation was imaged within adenosine 5′-diphosphate-loaded microcapillaries, revealing clearly visible patterns. This darkfield cellular light scatter approach was previously developed for bacterial cells, and here we adapted and optimized it for directly monitoring platelet aggregation. Capturing high-quality time-resolved images of platelets undergoing activation within microcapillaries allowed us to measure changes in light scattering in platelet-rich plasma that correspond with aggregation measured using conventional laboratory methods. This novel prototype system shows that this approach may have potential for use in large-scale studies of platelet function, combining simplicity with low-cost components and using a disposable dip-and-test microfluidic format.
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Sarıyer et al. (2026) studied Healthy donors (blood samples for device testing). Dark-Pi imaging system vs. Unstimulated samples and standard laboratory methods was evaluated on Detection of platelet aggregation via light scattering intensity (p=0.0078). The Dark-Pi microfluidic system successfully detected ADP-induced platelet aggregation through changes in cellular light scattering, offering a low-cost, label-free approach for platelet function analysis.
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