Key result
Flow chamber devices combined with microspotting and fluorescence microscopy enable high-throughput measurement of platelet activation and coagulation phenotyping in human blood.
Microfluidic flow chamber devices combined with microspotting and multi-color fluorescence microscopy offer high-throughput capabilities for complex platelet and coagulation phenotyping in human blood.
Microfluidic devices with multi-color imaging advance platelet research; leaves open standardization and clinical translation.
In recent years, considerable progress has been made in understanding the mechanisms involved in platelet activation during hemostasis and thrombosis. Parallel-plate flow chambers and other microfluidic devices have markedly contributed to this insight. Conversely, such flow devices are now increasingly used to monitor the combined processes of platelet aggregation, thrombus formation, and coagulation in human blood. Currently, by combining microspotting and multi-color fluorescence microscopy, this technology offers the capability of high-throughput measurement of platelet activation processes, even in small blood samples. Here we review the potential of flow chamber devices for complex (multiparameter) platelet and coagulation phenotyping, focusing on patients with (genetic) platelet- or coagulation-based bleeding disorders as well as monitoring of antithrombotic medication. Animal studies are not discussed.
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Brouns et al. (2018) conducted a review in Bleeding disorders. Flow chamber devices was evaluated. Flow chamber devices combined with microspotting and fluorescence microscopy enable high-throughput measurement of platelet activation and coagulation phenotyping in human blood.
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